Genes expected to be absent, or expressed at a notably low level, in these cells, and whose absence supports the assignment of this cell annotation.
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Rationale
This cell was annotated manually using canonical markers in the field (NOTCH3, PDGFRB, MYO1B, MYH11, TAGLN). We noticed TNC, DELEC1 running differential expression.
Lineage annotations were determined by Louvain clustering at resolution 0.1. Each lineage (fibroblast, muscle, immune, stromal) was then subsetted and reclustered. Final annotation resolution was chosen according to expression of biological markers; smaller clusters were combined were appropriate